The test microorganism is grown in liquid culture .
The concentration of the test microorganism is standardized .
The microbial culture is diluted in a sterile nutritive solution .
Control and test fabric swatches are inoculated with microorganisms.
The inoculation is performed such that the microbial suspension touches only the fabric ( see original method for details)
Bacteria levels on both control and test fabrics are determined at “ time zero” by elution in a large volume of neutralizing broth , followed by dilution and plating .
A control is run to verify that the neutralization /elution method effectively neutralizes the antimicrobial agent in the fabric.
Additional inoculated control and test fabrics are allowed to incubate , unditurbed in sealed jars , for 24 hours
After incubation , microbial , concentrations are determined
Reduction of microorganisms relative to initial concentrations and the control fabric is calculated .
Post time: Nov-28-2022